Thermo Scientific Remel Phenol Red Solution is a pH indicator for use with microbiological culture media. Its color exhibits a gradual transition from yellow to red over the pH range 6.8 to 8.2. '�.A��� ��m�B��-� p�����y��m@x�� ��3�*J>��#��F�D>R�)�@R��(�1� �#ʹR��RC0�7a�����D1��Q� �\S �IJ"7�sを�����\��D����f��w��$��W'��9���;�?�Ҿ�儝�)Z9���CD��� u�z�aXHH^� � Ԏ�e��6v��w8�.�R�h�I��%5� �Xh��G ����X�fcу�X 7�{=�ޛ���A�B ����+��oڑ�B@����HO���.���~�ٴ#��~��.�I�%¢����'N�䃂H;�v�wi�'�{IZ��B@�i=�큥٠�(y����G;�|�!=a@H�f�. Phenol red (also known as phenolsulfonphthalein or PSP) is a pH indicator frequently used in cell biology laboratories. Phenol Red, sodium salt. A solution of phenol red will have a yellow color at a pH of 6.4 or below and a red color at a pH… Standardized for colorimetric pH determination. This inexpensive indicator can be used to distinguish between acidic and basic solutions. Hence, the relative concentration of one form, which varies as a function of … Its solubility is 0.77 grams per liter (g/l) in water and 2.9 g/l in ethanol. A solution of phenol red is used as a pH indicator: its color exhibits a gradual transition from yellow to red over the pH range 6.6 to 8.0. Phenol red exists as a red crystal that is stable in air. Phenol Red sodium salt, ACS reagent. Above pH 8.2, phenol red turns a bright pink (fuchsia) color. Phenol red SDS Safety Data Sheet for Phenol red 107241. Phenol red salt is used routinely as pH indicator.
If the organism is able to utilize the carbohydrate, an acid by-product is created, which turns the media yellow. It is a weak acid with pKa= 8.00 at 20°C. Beilstein/REAXYS Number 326470 . DTXSID2044420. The pH of blood ranges from 7.35 to 7.45, for instance. DSSTox_RID_80214. %PDF-1.6
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use of color change of phenol red as the indicator in titrating poliomyelitis virus or its antibody in a tissue–culture system 1 2 Its color exhibits a gradual transition from yellow (λmax = 443 nm[2]) to red (λmax = 570 nm[3]) over the pH range 6.8 to 8.2. Phenol red is a weak estrogen mimic, and in cell cultures can enhance the growth of cells that express the estrogen receptor. Since the color of phenol red can interfere with some spectrophotometric and fluorescent assays, many types of tissue culture media are also available without phenol red. Molecular Weight 354.38 . SCHEMBL222482. [8], Several indicators share a similar structure to phenol red, including bromothymol blue, thymol blue, bromocresol purple, thymolphthalein, and phenolphthalein. grade. It is a weak acid with pK a = 8.00 at 20 °C (68 °F). Color range: pH 6.0-8.5. Most living tissues prosper at a near-neutral pH; that is, a pH close to 7. ?���3��.�ߞ�9;����?���VF�{�����Ƌ�wgW?�����woN/$������ӫӋ������O^\������o��IU����/W��N~���.�� CV�?�1��ɫ���!��D\ͣ�@�pz�H9����'�s����i EC Number 205-609-7. [1][7] However, this cyclic structure could not be confirmed by X-ray crystallography. HY-D0169A. The test is based on the fact that phenol red is excreted almost entirely in the urine. �8�z0E00�1�2:+0(�{0�0IU1�jPO`d�R`�a���ʰ����QoC��5�8y��[)p]`�����ɰ��"%�Кp;��?�i��H�c�; e�bVf[(�@� Р!#
Previous. PHENOL RED INDICATOR AR (pH INDICATOR) (PH 6.4-8.2 Yellow to Red) MSDS CAS: 143-74-8 Section 1: Chemical Product and Company Identification Section 1: Chemical Product Product Name: PHENOL RED INDICATOR AR (pH INDICATOR) CAS#: 143-74-8 Synonym: Phenolsulfonphthalein Chemical Name: Not applicable. For example, a culture of relatively slowly dividing mammalian cells can be quickly overgrown by bacterial contamination. February 28, 2019 admin Ingredients 0. For example, phenol red exhibits an orange color between pH 6.8 and pH 8.4. ���ɨ#�3#P[ƶ�.K�-CV�3v$N$p B�@�] �e8: �yP;P��C�Q?y��P�1 ����o�z�iЯ.����y�`�,�8{�m-b��yR!�7��
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�-���($`�9#@�pM�M�*�\�4��`�R����. That I am using is " Phenol Red indicator, ACS reagent, CAS Number 143-74-8 ". phenol red indicator. Phenol Red Indicator, ACS reagent, CAS Number: 143-74-8,(32661 Fluka).Shop now or request a quote. Indicators usually exhibit intermediate colors at pH values inside the listed transition range. DSSTox_GSID_44420. KS-00000XAO. (A table of other common chemical indicators is available in the article on pH indicators.). ,"]���n����S��^���`�*Xv*� ��`[5�f��U�
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In Vitro ADMET Laboratories, LLC. Bottle/50 mL spec. Polio (IPV – Ipol) Next. There are numerous formulations for in vitro cell propagation. Above pH 8.1, phenol red turns a bright pink (fuchsia) color. Calculations like this one might make a good project for a college freshman chemistry class. Phenol red exists as a red crystal that is stable in air. grade. Phenol Red Broth is a general-purpose differential test medium typically used to differentiate gram negative enteric bacteria.It contains peptone, phenol red (a pH indicator), a Durham tube, and one carbohydrate (glucose, lactose, or sucrose). When cells are grown in tissue culture, the medium in which they grow is held close to this physiological pH. Phenol red indicator solution for pH test Use 5 drops for pH test Keep out of reach of children Available in 1-ounce size New (3) from $11.99 + FREE Shipping. Phenol Red is used as a pH indicator in cell culture applications. 59 0 obj
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Gas Production 1. 3 The two forms of the dye have different optical absorption spectra. Congo Red (powder) C19H13NaO5S. Bovine Brain Endothelial Cell Growth Medium without Phenol Red (500 ml) 1 Product Result Principle: Phenol Red Fermentation medium contains peptone, phenol red (a pH indicator) and the carbohydrate to be tested. A solution of phenol red is used as a pH indicator: its color I. Phenolsulfonphthalein is being investigated for use clinically due to its weak estrogen mimicking actions. Acid production: 1. They are used to visually signal the acidity or alkalinity of an aqueous (water-based) solution. High levels of bromine can convert phenol red to bromophenol red (dibromophenolsulfonephthalein, whose lowered pKa results in an indicator with a range shifted in the acidic direction – water at pH 6.8 will appear to test at 7.5). pH Indicator Ranges (Ascending pH) Acid-base pH indicators are solutions that have a characteristic color at certain pH levels. MDL number MFCD00003552 Many biologists find this a convenient way to rapidly check on the health of tissue cultures. Even higher levels of bromine (>20 ppm) can result in the secondary conversion of bromophenol red to bromophenol blue with an even lower pKa, erroneously giving the impression that the water has an extremely high pH despite being dangerously low. EINECS 252-057-8. At still higher pH (pKa = 7.7), the phenol's hydroxy group loses its proton, resulting in the red ion denoted as PS2−. After the solution is effected, add sufficient water to produce 100 ml. Bottle/50 mL spec. These items are shipped from and sold by different sellers. Formula: C₁₉H₁₄O₅S MW: 354.38 g/mol Storage Temperature: Ambient: MDL Number: MFCD00003552 CAS Number: 143-74-8: Order Now. It is provided as an optional media component when preparing complete media as part of a complete ATCC … h��[k����+��F"l�M�=,Kε�H���6��Y`�5�u�����T�=�=3;;�R^��f�XUdUž�h:Ӈ.����E��/ߙ������r����d��+(�}��KΡK��>�.Y��"�.�T����R�P
�Cz���DH]ʜ�Gm�GS��#�3z��R`�JE�cJ}� L���&c&l�2zN�!���z�}�]A��t=g���\ The transition range may shift slightly depending on the concentration of the indicator in the solution and on the temperature at which it is used. Albert P Li. Phenolsulfonphthalein or otherwise called phenol red is a pH indicator commonly used in cell biology laboratories. Phenol red is an acid-base indicator. Frequently bought together + + Total price: $29.48. Many of these defined mixtures also contain phenol red. Find chemicals for your experiments at Home Science Tools! h�b``�a``�b 'P�0p4p ��@1C � �(��u Phenol red has been used as a pH indicator dye in tissue culture media for decades (Figure 1). MW 354.37. pH indicator widely used in culture media to identify pH changes from neutral (red) to acidic (yellow) values. Empirical Formula (Hill Notation) C 19 H 14 O 5 S . A small amount of phenol red added to this growth medium will have a pink-red color under normal conditions. Phenol red is an indicator commonly used to measure pH in swimming pool test kits, see e.g. This form is sometimes symbolically written as H+2PS− and is orange-red. 2. This color change is an indication that even in the absence of contamination, the medium needs to be replaced (generally, this should be done before the medium has turned completely orange). Phenol red solution is administered intravenously, the urine produced is collected. Glycerin. Typically, 15 mg/l are used for cell culture. Phenol Red, sodium salt, pure, water soluble, indicator. Depending on the intended cell type different mixtures of salts, amino acids, vitamins, buffers, carbohydrates, fatty acids, and supplements such as serum are used. Additionally, phenol red tubes contain an inverted Durham tube. Its solubility is 0.77 grams per liter (g/L) in water and 2.9 g/L in ethanol. �]���{̧$�S"�X�)�S�L`ͥt����2�k0H9N���uE��$�Rt� u��b �-�R�q I�EA�rwL��5� �@c��Q����L8��hY�r���ɱ-�H��#s�1=c1 ��X�ao1�5���d)�@�v��ɣ���<9��������s��ɣ�(���O�������G�]��2��jϿ������+�J�ļ�O���Z� When cells are grown in tissue culture, the medium in which they grow is held close to this physiological pH. Phenol Red Indicator Structure Search; Print SDS. h�bbd```b``>"@$S�T�
"Y�"���`�"0��^ �"�R@$W&X6,k fk�H�H)} More information about phenol red indicator in vaccines coming soon. Phenol red is a pH indicator which turns yellow below a pH of 6.8 and fuchsia above a pH of 7.4. Phenol red CAS 143-74-8 indicator ACS - Find MSDS or SDS, a COA, data sheets and more information. Phenol red is a pH indicator. Phenol red exists as a red crystal that is stable in air. A small amount of phenol red added to this growth medium will have a pink-red color under normal conditions. This usually results in an acidification of the medium, turning it yellow. %%EOF
Phenol Red Indicator Solution: Dissolve 0.1 g of phenol red in 2.82 ml of 0.1 M sodium hydroxide and 20 ml of ethanol (95 percent). [4][5], In crystalline form, and in solution under very acidic conditions (low pH), the compound exists as a zwitterion as in the structure shown above, with the sulfate group negatively charged, and the ketone group carrying an additional proton. [12], Phenol red, sometimes labelled with a different name, such as "Guardex Solution #2", is used as a pH indicator in home swimming pool test kits. A solution of phenol red is used as a pH indicator, often in cell culture. [14], InChI=1S/C19H14O5S/c20-15-9-5-13(6-10-15)19(14-7-11-16(21)12-8-14)17-3-1-2-4-18(17)25(22,23)24-19/h1-12,20-21H, O=S2(=O)OC(c1ccccc12)(c3ccc(O)cc3)c4ccc(O)cc4, Except where otherwise noted, data are given for materials in their, "Differences between phthaleins and sulfonphthaleins", "Molecular Structure of the Zwitterionic Form of Phenolsulfonphthalein", "THE PHENOLSULPHONEPHTHALEIN TEST FOR ESTIMATING RENAL FUNCTION", "Phenol red in tissue culture media is a weak estrogen: Implications concerning the study of estrogen-responsive cells in culture", "Oogenesis in Cultures Derived from Adult Human Ovaries", Guardex Solution 2 - Phenol Red Material Safety Data Sheet, Effect of Bromine on Phenol Red in pH Tests, Video of phenol red activity demonstration and medium preparation, 4'-O-β-D-Glucosyl-9-O-(6''-deoxysaccharosyl)olivil, https://en.wikipedia.org/w/index.php?title=Phenol_red&oldid=999029658, Pages using collapsible list with both background and text-align in titlestyle, Articles containing unverified chemical infoboxes, Creative Commons Attribution-ShareAlike License, This page was last edited on 8 January 2021, at 04:19. 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